Flow Cytometry Stem Cell Enumeration

Cytokine and chemokine profiling.

Flow cytometry stem cell enumeration.

Bd stem cell enumeration starter kit includes software for first time users. For the analysis of cd34 hscs cell samples are stained with fluorescently labeled antibodies against both the hsc antigen cd34 and the pan leukocyte antigen cd45. Flow cytometric enumeration of cd34 cells is commonly employed to assess the hematopoietic stem cell hsc numbers in peripheral blood cord blood and apheresis products used for peripheral blood stem cell pbsc transplantation. Issues in enumeration applications of flow cytometry in stem cell research and tissue regeneration 10 1002 9780470631119 115 134 2012.

Several flow cytometric studies have shown. Multicolor flow cytometry stem cell research t cell research support support sales and support faq technical support training resources and tools. Immunophenotyping of blood products. Centers for disease control.

Some of the cytometry tests we offer. Hematopoietic progenitor cells or hpcs are used to reconstitute hematopoiesis following marrow ablative therapies. Enumeration of cd34 cells in cord blood. The cd34 antigen is stage specific and identifies cells in the early stages of hematopoietic differentiation.

Detection of minimal residual disease. Identification of cd34 hematopoietic stem cells cd34 cell enumeration by flow cytometry is a rare event analysis. In the present study we review and compare the major counting techniques of stem and progenitor cells. The advent and use of a cd34 monoclonal antibody enabled the detection of these cells by flow cytometry.

I am a development technologist for the human cellular therapy laboratory at mayo clinic. Flow cytometric enumeration of cd34 hematopoietic stem and progenitor cells hscs is widely used for evaluation of graft adequacy of peripheral blood and bone marrow stem cell grafts. A variation on a single platform flow cytometric method based on the ishage gating strategy. Furthermore cd34 cell enumeration is useful for monitoring the stem cell yield during graft manipulations such as immunomagnetic selections ex vivo expansion or genetic modifications of hscs.